Comparison between the Serological and Molecular Methods for the Detection of Helicobacter pylori
DOI:
https://doi.org/10.59675/U311Keywords:
Helicobacter pylori, Gram-negative helical bacteria, gastrointestinal mucosa , Elisa IgM, IgG. PCRAbstract
Background: Helicobacter pylori (H. pylori) infection is a worldwide health issue associated with chronic gastritis, stomach ulcers, and neoplasms. Management requires an accurate diagnosis, and the currently available technologies can be divided into molecular and serological approaches. Every approach has advantages and disadvantages, highlighting the importance of comparing.
Objectives: This study aimed to evaluate the diagnostic efficiency, reliability, and usefulness of serological and molecular methods for detecting H. pylori infection.
Methods: In 2023, a cross-sectional study was performed in Wasit Province, Iraq. Five hundred forty-one volunteers aged 15 to 65 years showing dyspeptic symptoms submitted blood samples for analysis. Serological testing utilized ELISA to identify IgG and IgM antibodies, whereas PCR was performed for the molecular detection of H. pylori DNA. Data were analyzed to assess sensitivity, specificity, and demographic correlations. The prevalence of seasonal swings was also evaluated.
Results: The frequency of H. pylori was nearly the same across males (270) and females (271). The most considerable prevalence was observed in the 26–35 age group, with seasonal peaks during the summer, especially in July. PCR had superior sensitivity and specificity relative to serology, although it required advanced resources. Serological tests offered significant epidemiological findings, although they could not differentiate between active and past infectious diseases.
Conclusion: Serological tests are useful for epidemiological investigations, but molecular methods are more accurate. A combined diagnostic strategy improves clinical and public health measures, especially in resource-limited regions. Seasonal and demographic changes require specific measures.
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References
Hunt R, Xiao S, Megraud F, Leon-Barua R, Bazzoli F, Van der Merwe S, et al. Helicobacter Pylon in Developing Countries. World Gastroenterology Organisation Global Guideline. Journal of Gastrointestinal & Liver Diseases. 2011;20(3).
Salem E, Sakr A, Younis F, Mohamed A. Prevalence of Helicobacter pylori Infection among farmers and non-farmers with dyspepsia. Egypt J Occup Med. 2019;43(2):229-44. DOI: https://doi.org/10.21608/ejom.2019.31419
Fagoonee S, Pellicano R. Helicobacter pylori: molecular basis for colonization and survival in gastric environment and resistance to antibiotics. A short review. Infectious Diseases. 2019;51(6):399-408. DOI: https://doi.org/10.1080/23744235.2019.1588472
Kao C-Y, Sheu B-S, Wu J-J. Helicobacter pylori infection: An overview of bacterial virulence factors and pathogenesis. Biomedical journal. 2016;39(1):14-23. DOI: https://doi.org/10.1016/j.bj.2015.06.002
Mladenova-Hristova I, Grekova O, Patel A. Zoonotic potential of Helicobacter spp. Journal of Microbiology, Immunology and Infection. 2017;50(3):265-9. DOI: https://doi.org/10.1016/j.jmii.2016.11.003
Ranjbar R, Chehelgerdi M. Genotyping and antibiotic resistance properties of Helicobacter pylori strains isolated from human and animal gastric biopsies. Infection and drug resistance. 2018:2545-54. DOI: https://doi.org/10.2147/IDR.S187885
El-Gohary AH, Yousef MA, Mohamed AA, El-Amaiem W, Abdel-Kareem LM. Epidemiological study on H. pylori in cattle and its milk with special reference to its zoonotic importance. Biol Med. 2015;7(5):1. DOI: https://doi.org/10.4172/0974-8369.1000251
Talaat Al Sherief LM, Thabet SS. Isolation of Helicobacter pylori from Raw Milk and Study on Its Survival in Fermented Milk Products. Journal of Applied Veterinary Sciences. 2022;7(2):73-81. DOI: https://doi.org/10.21608/javs.2022.124671.1133
Canejo-Teixeira R, Oliveira M, Pissarra H, Niza MMR, Vilela CL. A mixed population of Helicobacter pylori, Helicobacter bizzozeronii and “Helicobacter heilmannii” in the gastric mucosa of a domestic cat. Irish veterinary journal. 2014;67:1-4. DOI: https://doi.org/10.1186/2046-0481-67-25
Yokota K, Osaki T, Hayashi S, Yokota Si, Takeuchi H, Rimbara E, et al. Establishment of a reference panel of Helicobacter pylori strains for antimicrobial susceptibility testing. Helicobacter. 2022;27(3):e12874. DOI: https://doi.org/10.1111/hel.12874
Parente J, Da Silva BB, Palha-Dias M, Zaterka S, Nishimura NF, Zeitune JM. Helicobacter pylori infection in children of low and high socioeconomic status in northeastern Brazil. The American journal of tropical medicine and hygiene. 2006;75(3):509-12. DOI: https://doi.org/10.4269/ajtmh.2006.75.509
Ricci C, Holton J, Vaira D. Diagnosis of Helicobacter pylori: invasive and non-invasive tests. Best Practice & Research Clinical Gastroenterology. 2007;21(2):299-313. DOI: https://doi.org/10.1016/j.bpg.2006.11.002
Best LM, Takwoingi Y, Siddique S, Selladurai A, Gandhi A, Low B, et al. Non‐invasive diagnostic tests for Helicobacter pylori infection. Cochrane Database of Systematic Reviews. 2018(3). DOI: https://doi.org/10.1002/14651858.CD012080.pub2
Patel SK, Pratap CB, Jain AK, Gulati AK, Nath G. Diagnosis of Helicobacter pylori: what should be the gold standard? World journal of gastroenterology: WJG. 2014;20(36):12847. DOI: https://doi.org/10.3748/wjg.v20.i36.12847
Hirschl AM, Makristathis A. Methods to detect Helicobacter pylori: from culture to molecular biology. Helicobacter. 2007;12:6-11. DOI: https://doi.org/10.1111/j.1523-5378.2007.00560.x
Stefano K, Rosalia A, Chiara B, Federica G, Marco M, Gioacchino L, et al. Non-invasive tests for the diagnosis of helicobacter pylori: state of the art. Acta Bio Medica: Atenei Parmensis. 2018;89(Suppl 8):58.
Kusters J, van Vliet A, Kuipers E. Pathogenesis of Helicobacter pylori Infection Clin Microbiol Rev 19: 449-490. Crossref Google Scholar. 2006. DOI: https://doi.org/10.1128/CMR.00054-05
Kazemi S, Tavakkoli H, Habizadeh MR, Emami MH. Diagnostic values of Helicobacter pylori diagnostic tests: stool antigen test, urea breath test, rapid urease test, serology and histology. Journal of research in medical sciences: the official journal of Isfahan University of Medical Sciences. 2011;16(9):1097.
Bénéjat L, Ducournau A, Lehours P, Mégraud F. Real‐time PCR for Helicobacter pylori diagnosis. The best tools available. Helicobacter. 2018;23(5):e12512. DOI: https://doi.org/10.1111/hel.12512
Zhu F, Zhang X, Li P, Zhu Y. Effect of Helicobacter pylori eradication on gastric precancerous lesions: A systematic review and meta‐analysis. Helicobacter. 2023;28(6):e13013. DOI: https://doi.org/10.1111/hel.13013
Bocu R. Extended Review Concerning the Integration of Electrochemical Biosensors into Modern IoT and Wearable Devices. Biosensors. 2024;14(5):214. DOI: https://doi.org/10.3390/bios14050214
Malfertheiner P, Megraud F, O'Morain CA, Atherton J, Axon AT, Bazzoli F, et al. Management of Helicobacter pylori infection—the Maastricht IV/Florence consensus report. Gut. 2012;61(5):646-64. DOI: https://doi.org/10.1136/gutjnl-2012-302084
Jemilohun AC, Otegbayo JA. Helicobacter pylori infection: past, present and future. Pan African Medical Journal. 2016;23(1). DOI: https://doi.org/10.11604/pamj.2016.23.216.8852
Ueda J, Okuda M, Nishiyama T, Lin Y, Fukuda Y, Kikuchi S. Diagnostic accuracy of the E-plate serum antibody test kit in detecting Helicobacter pylori infection among Japanese children. Journal of epidemiology. 2014;24(1):47-51. DOI: https://doi.org/10.2188/jea.JE20130078
Rimbara E, Sasatsu M, Graham DY. PCR detection of Helicobacter pylori in clinical samples. PCR Detection of Microbial Pathogens. 2013:279-87. DOI: https://doi.org/10.1007/978-1-60327-353-4_19
Duś I, Dobosz T, Manzin A, Loi G, Serra C, Radwan-Oczko M. Role of PCR in Helicobacter pylori diagnostics and research--new approaches for study of coccoid and spiral forms of the bacteria. Postepy Hig Med Dosw (Online). 2013;67(9):261-18. DOI: https://doi.org/10.5604/17322693.1044005
Saez J, Belda S, Santibáñez M, Rodríguez JC, Sola-Vera J, Galiana A, et al. Real-time PCR for diagnosing Helicobacter pylori infection in patients with upper gastrointestinal bleeding: comparison with other classical diagnostic methods. Journal of clinical microbiology. 2012;50(10):3233-7. DOI: https://doi.org/10.1128/JCM.01205-12
Lehours P, Mégraud F. Helicobacter pylori molecular diagnosis. Expert review of molecular diagnostics. 2011;11(4):351-5. DOI: https://doi.org/10.1586/erm.11.17
Schweizer HP. Understanding efflux in Gram-negative bacteria: opportunities for drug discovery. Expert opinion on drug discovery. 2012;7(7):633-42. DOI: https://doi.org/10.1517/17460441.2012.688949
Peleteiro B, Bastos A, Ferro A, Lunet N. Prevalence of Helicobacter pylori infection worldwide: a systematic review of studies with national coverage. Digestive diseases and sciences. 2014;59:1698-709. DOI: https://doi.org/10.1007/s10620-014-3063-0
Hong W, Tang H, Dong X, Hu S, Yan Y, Basharat Z, et al. Prevalence of Helicobacter pylori infection in a third-tier Chinese city: relationship with gender, age, birth-year and survey years. Microb Health Dis. 2019;1:e150.
De Martel C, Parsonnet J. Helicobacter pylori infection and gender: a meta-analysis of population-based prevalence surveys. Digestive diseases and sciences. 2006;51:2292-301. DOI: https://doi.org/10.1007/s10620-006-9210-5
Moshkowitz M, Konikoff F, Arber N, Peled Y, Santo M, Bujanover Y, et al. Seasonal Variation in the Frequency ofHelicobacter pylori Infection: A Possible Cause of the Seasonal Occurrence of Peptic Ulcer Disease. American Journal of Gastroenterology (Springer Nature). 1994;89(5).
Hooi JK, Lai WY, Ng WK, Suen MM, Underwood FE, Tanyingoh D, et al. Global prevalence of Helicobacter pylori infection: systematic review and meta-analysis. Gastroenterology. 2017;153(2):420-9. DOI: https://doi.org/10.1053/j.gastro.2017.04.022
Lames H. Almansee, Sumaya Mohammad Obayies, Ban Abbas Kareem, & Husam Hosny. The Role of Helicobacter Pylori in Chronic Tonsillitis. Academic International Journal of Medical Sciences ,2024 2(2), 32-38. https://doi.org/10.59675/M226 DOI: https://doi.org/10.59675/M226
Fadhl ALzamili, & Nisreen Jawad Kadhim. Diagnosis Study of Peptic Ulcer in Iraqi Patients Students. Academic International Journal of Medical Sciences 2024 ,2(2), 12-19. https://doi.org/10.59675/M223. DOI: https://doi.org/10.59675/M223
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