Association the Level of Glutathione S-Transferases with GST Genotyping in Automotive Maintenance Workers
DOI:
https://doi.org/10.59675/U227Keywords:
GST, Null Genotyping, Automotive Maintenance workers.Abstract
The present study aims to investigate the GST level with GST null genotyping in automotive maintenance workers; null genotyping's PCR and PCR-SSCP were used in the present study, including GSTT1, GSTM1, and GSTP1) with detection of GST level in the serum. The present study found sig changes in the mean of age between groups (p 0.000), nonsig changes in BMI, and non-sig changes in elevation in GSH between groups; the work duration was (7.68±4.88) years. The results of the present study show that (37.5%) of cases have deletion mutation in GSTT1 in significant association (p 0.0495). In contrast, the deletion mutation in GSTM1 was not observed in study groups. The haplotypes of GSTP1 found single and double haplotypes, both haplotypes observed in cases and control group in non-sig differences (p 0.8548); the correlation between GST level with age, BMI and duration showed weak inverse correlation (r -0.141, p 0.434) with age. a Weak positive correlation with duration (r 0.099, p 0.589) and a weak inverse correlation with BMI (r -0.046, p 0.798) in the case group. In the control group, a weak inverse relation with age (r -0.280, p 0.157) and a weak inverse relation with BMI (r -0.131, p 0.514). According to GST gene genotyping, the GST levels were shown to slightly elevate the GST level in the group with GSTT1 deletion in case of non-sig differences (p 0.475). The slight changes between case and control in non-significantly (p 0.247), no significant changes between the groups have normal GSTM1 (p 0.093), the output referred to elevation GST level in the case with single haplotype in non-sig differences (p 0.094), others showed slight changes in non-significant differences also, conclusion: we can conclude that the GSTT1 was a strong association with an automotive technician but GSTM1 and GSTP1 have no affected in this employee, on the other hand, the GST level don't changes by this employee, also didn't affect by GST genotypes.Downloads
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